| RBRC No. | RBRC06313 |
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| Type | Targeted Mutation |
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| Species | Mus musculus | ||||||||||
| Strain name | B6.129-Sparc<tm1Hwe> | ||||||||||
| Former Common name | B6-Sparc KO | ||||||||||
| H-2 Haplotype | No Data | ||||||||||
| ES cell line | CCE/EK.CCE [129S/SvEv-Gpi1 |
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| Background strain | No Data | ||||||||||
| Appearance | |||||||||||
| 1 | Appearance | No Data | |||||||||
| Genotype | No Data | ||||||||||
| Strain development | Developed by Chin Chen Howem The Wistar Institute. CCB ES cells were used to generate the knockout mice. The mutant mice were maintained by Yasumasa Kato, Ohu university School of Dentistry through Helene Sga, Benaroya Research Institute at Virginia. C57BL/6 congenic. | ||||||||||
| Strain description | Sparc (osteonectin, BM-40) gene knockout mice. Exon 4 of Sparc gene was replaced with MC1-neo and Pol2-neo tandem cassettes. Homozygous mutant mice exhibit cataracts, abnormal skeletal morphology and osteopenia (viable and fertile). | ||||||||||
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] | ||||||||||
| Health Report |
BRC facility Health Report in the last year and a half
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| Gene Details | |||||||||||
| Promoter | No Data | ||||||||||
| 1 | Symbol | GH | |||||||||
| Symbol name | Growth hormone polyA (Bovine) | ||||||||||
| Chromosome | 11 | ||||||||||
| Common name | No Data | ||||||||||
| Symbol description | No Data | ||||||||||
| 2 | Symbol | Sparc | |||||||||
| Symbol name | secreted acidic cysteine rich glycoprotein | ||||||||||
| Chromosome | 11 | ||||||||||
| Common name | No Data | ||||||||||
| Symbol description | No Data | ||||||||||
| Promoter | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||||||||
| 3 | Symbol | neo | |||||||||
| Symbol name | neomycin resistance gene (E. coli) | ||||||||||
| Chromosome | 11 | ||||||||||
| Common name | No Data | ||||||||||
| Symbol description | No Data | ||||||||||
| Promoter | mouse RNA polymerase II promoter | ||||||||||
| 4 | Symbol | neo | |||||||||
| Symbol name | neomycin resistance gene (E. coli) | ||||||||||
| Chromosome | 11 | ||||||||||
| Common name | No Data | ||||||||||
| Symbol description | No Data | ||||||||||
| References | |||||||||||
| Research applications | Cancer Research, Cell Biology Research, Developmental Biology Research, Hematological Research |
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| Specific Term and Conditions | The following terms and conditions will be requested by the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998 ). |
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| Additional information | |||||||||||
| 1 | No Data | ||||||||||
| 2 | Genotyping protocol <PCR> | ||||||||||
| Depositor | Kato, Yasumasa (Kanagawa Dental College)  |
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| Strain Status / Availability (Expected delivery) |
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