RBRC06315, MF1.129-Sparc<tm1Cam>

RBRC No. RBRC06315
Type Targeted MutationCartagena
Species Mus musculus
Strain name MF1.129-Sparc<tm1Cam>
Former Common name MF1-Sparc KO
H-2 Haplotype No Data
ES cell line CCB [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by William H. Colledge, University of Cambridge. CCB ES cells were used. Maintained by Yasumasa Kato, Ohu university School of Dentistry. C3H congenic (over N9 generation) (RBRC06314), MF1 mixed background (RBRC06315).
Strain description Sparc (osteonectin, BM-40) gene knockout mice. Exon 6 of Sparc gene was replaced with a SA-lacZ/PGK-neo cassette. Homozygous mutant mice exhibit cataracts.
Colony maintenance Homozygote x Homozygote [or Crossing to HsdOla:MF1]
Health Report
Gene Details
Promoter No Data
1 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol Sparc
Symbol name secreted acidic cysteine rich glycoprotein
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Hematological Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EMBO J., 17, 1860-1870 (1998).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Yasumasa (Kanagawa Dental College) Kato, Yasumasa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months