RBRC06336, NOD.Cg-Prkdc<scid> E2f1<tm1Meg>

RBRC No. RBRC06336
Type Targeted Mutation and Spontaneous Mutation CongenicCartagena
Species Mus musculus
Strain name NOD.Cg-Prkdc<scid> E2f1<tm1Meg>
Former Common name NOD.e2f1<+/->, NOD-scid/E2f1 KO
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Hidenobu Senpuku, National Institute of Infectious Diseases. The E2f1<tm1Meg> mice of C57BL/6 and 129 mixed background were backcrossed to NOD/LtJ mice, and furthermore the mutant mice were backcrossed to NOD/SCID mice.
Strain description E2f1 deficient NOD/SCID mice. Part of exon 3 to exon 4 of E2f1 gene was replaced with a neo cassette. E2f1 homozygous mutant mice are immunodeficiency and show decreased saliva production.
Colony maintenance E2f1: Heterozygote x Wild-type; scid: Homozygote x Homozygote [or Crossing to NOD/ShiJic-scid/Jcl]
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
May 14, 2018 2-検 F
Mar 12, 2018 2-検 F
Jan 15, 2018 2-検 F
Nov 13, 2017 2-検 F
Sep 11, 2017 2-検 F
Jul 10, 2017 2-検 F
May 15, 2017 2-検 F
Mar 13, 2017 2-検 F

Gene Details
Promoter No Data
1 Symbol Prkdc
Symbol name protein kinase, DNA activated, catalytic polypeptide
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol E2f1
Symbol name E2F transcription factor 1
Chromosome 2
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name No Data
Symbol description No Data

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Exp. Biol. Med. (Maywood)., 234, 1525-1536 (2009).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Senpuku, Hidenori (National Institute of Infectious Diseases) Senpuku, Hidenori
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months