RBRC06350, B6.129S2-Asf1b<Gt(ROSABetageo)437Muv>/Skmz

RBRC No. RBRC06350
Type Gene Trap
Species Mus musculus
Strain name B6.129S2-Asf1b<Gt(ROSABetageo)437Muv>/Skmz
Former Common name B6-CIA-II, B6-Asf1b Gt
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively.
Strain description CIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Asf1b
Symbol name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol LTR
Symbol name retrovirus LTR (viral long terminal repeat)
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 8
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
References Genes Dev. 1991 Sep;5(9):1513-23.

Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Research applications Cancer Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定。 (will be announced).
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Sekimizu, Kazuhisa (The University of Tokyo) Sekimizu, Kazuhisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months