Strain Data Sheet

RBRC06412

Strain Information

Image
BRC No.RBRC06412
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Wtap<Gt(OST200529)Lex>
Former Common nameWtap KO, Wtap Gt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takao Hamakubo, Research Center for Advanced Science and Technology, University of Tokyo. C57BL/6 and 129 mixed background.
Strain descriptionWtap gene gene-trap mice. Homozygous mutant mice are embryonic lethal (around E6.5).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6J(Crlj)]
ReferencesProc. Natl. Acad. Sci. USA, 103, 17278-17283 (2006). 17088532

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WtapWilms tumour 1-associating protein17Wtap<Gt(OST200529)Lex>gene trap OST178967, Lexicon Genetics

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BTKBurton's tyrosine kinase17BTK

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
LTRretrovirus LTR (viral long terminal repeat)17LTR

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal17

Ordering Information

Donor DNAretrovirus　LTR, マウス PGK promoter, ヒト BTK exon, simian virusSV40 polyA, 放線菌 Neomycin, 大腸菌LacZ
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 103, 17278-17283 (2006).
DepositorTakao Hamakubo (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data