RBRC06427, B6.Cg-Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur>

RBRC No. RBRC06427
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur>
Former Common name Rosa26-CAG-STOP-SCAT1, B6-ROSA26/SCAT1floxed KI
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ting Liu, Yoshifumi Yamaguchi and Masayuki Miura, Graduate School of Pharmaceutical Sciences, University of Tokyo, and Katsuaki Hoshino and Tsuneyasu Kaisho, RIKEN Research Center for Allergy and Immunology. C57BL/6J and C57BL/6N mixed.
Strain description CAG-STOP-CAST1 knock-in mice. A CAG-loxP-neo-loxP-SCAT1-pA cassette was inserted into the Gt(ROSA)26Sor locus. Homozygous mice are viable (fertility is unknown).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol ECFP
Symbol name Enhanced Cyan Fluorescent Protein (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References Liu et al., Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response Cell Reports, in press

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response" Cell Reports, 8(4):974-982 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
Depositor Miura, Masayuki (The University of Tokyo) Miura, Masayuki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months