Strain Information | |
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Image | |
BRC No. | RBRC06522 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Cthrc1<tm1.1Suna> |
Former Common name | Cthrc1 flox, B6-Cthrc1 floxed |
H-2 Haplotype | |
ES Cell line | iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Sunao Takeshita, National Center for Geriatrics and Gerontology. (C57BL/6NTac x 129S6/SvEvTac)F1 derived ES cells were used to generate the mutant mice. C57BL/6 background. Floxed mice (RBRC06522): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice. KO mice (RBRC06523): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice, and then the exon 2 was removed by crossing with CAG-Cre mice. |
Strain description | Cthrc1 gene floxed mice (RBRC06522)(Exon 2 was flanked by loxP sites). Cthrc1 gene KO mice (RBRC06523)(Exon 2 was deleted). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | J Clin Invest., 123, 3914-3924 (2013). 23908115 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cthrc1collagen triple helix repeat containing 115Cthrc1<tm1.1Suna>targeted mutation 1.1, Sunao Takeshita Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) site, P1 phage loxP sites, Mouse Cthrc1 gene |
Research application | Cancer Research Cre/loxP system FLP/frt system Hematological Research Metabolism Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin. Invest., 123, 3914-3924 (2013). |
Depositor | Kyoji Ikeda (National Center for Geriatrics and Gerontology) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |