RBRC06587, C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.2Imayo

RBRC No. RBRC06587
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.2Imayo
Former Common name R26 BAC::pCAG-FRT-ArchT-EGFP, B6-ROSA26/ArchT-GFP/Frt Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain description ArchT-EGFP is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26 BAC. This modified BAC was used for Tg mouse production. To achieve high-level transgene expression, ArchT-EGFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region. R26 BAC::pCAG-LF-ArchT-EGFP line (RBRC06585), R26 BAC::pCAG-LoxP-ArchT-EGFP line (RBRC06586) (FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain), R26 BAC::pCAG-FRT-ArchT-EGFP line (RBRC06587) (LoxP-Stop-LoxP cassette was removed by crossing TNAP-Cre strain).
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name beta-globin poly A signal (Rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome UN
Common name No Data
Symbol description No Data
6 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome UN
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
8 Symbol No Data
Symbol name ArchT (Halorubrum)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months