RBRC09239, ICR;129(Cg)-Zeb2<tm2.1Yhi>

RBRC No. RBRC09239
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR;129(Cg)-Zeb2<tm2.1Yhi>
Former Common name ICR-SIP1-EGFP, ICR-Zfhx1b/GFP KI
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yujiro Higashi, Aichi Human Service Center in 2008. R1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice (B6). ICR mixed background (RBRC09239), ICR and C57BL/6 mixed background (RBRC09240).
Strain description SIP1 (Zeb2, Zfhx1b) gene GFP knock-in mice. A EGFP was inserted in-frame with the coding sequence in exon 9 of the Zeb2 gene.
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Zeb2
Symbol name zinc finger E-box binding homeobox 2
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Mouse of the Month Jul 2015
3 Genotyping protocol <PCR>
Depositor Higashi, Yujiro (AICHI HUMAN SERVICE CENTER) Higashi, Yujiro
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months