RBRC09241, HR1.Cg-Hr<hr> Tg(EF1A-PDHL/roGFP1)121Soht

RBRC No. RBRC09241
Type Transgene Congenic and Spontaneous MutationCartagena
Species Mus musculus
Strain name HR1.Cg-Hr<hr> Tg(EF1A-PDHL/roGFP1)121Soht
Former Common name Mito-roGFP(MROH121), HR1-hr/Mito-roGFP
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Alexander M. Wolf, Nippon Medical School in 2012. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mutant mice were backcrossed to Hos:HR-1 over 10 generations.
Strain description Transgenic mice expressing ratiometric redox-sensitive GFP (roGFP) in the mitochondria of several tissues, including skin epidermal keratinocytes. Hairless mice background.
Colony maintenance Tg: Carrier x Noncarrier; hr: Homozygote x Homozygote [or Crossing to Hos:HR-1]
Health Report
Gene Details
Promoter No Data
1 Symbol Hr
Symbol name hairless
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol CSF3
Symbol name human G-CSF cDNA PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol roGFP
Symbol name Redox-sensitive Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human elongation factor 1 alpha (EF-1 alpha) promoter
4 Symbol PDHA1
Symbol name mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Dermatology Research,
Fluorescent Proteins/lacZ System,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Invest. Dermatol.,134(6), 1701-1709 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Ohta, Shigeo (Nippon Medical School) Ohta, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months