Strain Data Sheet

RBRC09245

Strain Information

Image
BRC No.RBRC09245
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ehmt2<tm2Yshk>
Former Common nameG9a-flox, B6-Ehmt2 floxed
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Makoto Tachibana and Yoichi Shinkai, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used to generate the mutant mice. C57BL/6 mixed background.
Strain descriptionG9a (Ehmt2) gene floxed mice. Exon 26, an exon 27/28 (cDNA) and a PGK-neo cassette were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6]
ReferencesEMBO J., 26, 3346-3359 (2007). 17599069

Health Report

Examination Date / Room / Rack2022/04/25Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ehmt2euchromatic histone lysine N-methyltransferase 217Ehmt2<tm2Yshk>targeted mutation 2, Yoichi Shinkai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ehmt2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J, 26, 3346-3359 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYoichi Shinkai (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data