RBRC09256, B6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa>

RBRC No. RBRC09256
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa>
Former Common name B6-ROSA/kikGR KI, B6-ROSA/kikGR KI
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Michio Tomura, Kyoto University Graduate School of Medicine. M1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice. C57BL/6 congenic.
Strain description green-to-red photoconvertible fluorescence protein, KikGR ROSA26 knock-in mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol kikGR
Symbol name Favia favus Kikume-Green Red cDNA
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. Osaka University (FAX:+81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). In publishing the research results to be obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tomura M et.al, Sci. Rep. e6030 (2014). The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT’s publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Necessary documents for ordering: 1. Approval form (form D) from the depositor (ENG) , (JPN) , 2. Approval form (form V) from the developer, 3. MTA for pCAGGS with the developer (Word) , 4. MTA for distribution with RIKEN BRC and 5. Order form (ENG) , (JPN) .
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :