RBRC09422, FVB.129S6-Gt(ROSA)26Sor<tm1(Pik3ca*E545K)Egan>

RBRC No. RBRC09422
Type Targeted MutationCartagena
Species Mus musculus
Strain name FVB.129S6-Gt(ROSA)26Sor<tm1(Pik3ca*E545K)Egan>
Former Common name R26-Pik3ca(E545K)
H-2 Haplotype No Data
Background strain FVB/NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development W4 mESC were transformed with linearized pROSA-Pik3ca-H1047R or pROSA-Pik3ca-E545K plasmid; this plasmid was built from pBIGT and pROSA gene targeting vectors and contained the mouse Pik3ca cDNA with H1047R or E545K point mutation.
Strain description Some mice develop blood vessel lesions.
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name trimer of the SV40 polyadenylation sequence (tpA)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Pik3ca
Symbol name phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must prior contact with the developer scientist(Dr. Sean E. Egan, segan@sickkids.ca, Hospital for Sick Children) and must obtain a use license.
Additional information
1 No Data
Depositor Okazaki, Yasuji (Saitama medical school research center for genomic medicine) Okazaki, Yasuji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months