Strain Data Sheet

RBRC09422

Strain Information

Image
BRC No.RBRC09422
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameFVB.129S6-Gt(ROSA)26Sor<tm1(Pik3ca*E545K)Egan>
Former Common nameR26-Pik3ca(E545K)
H-2 Haplotype
ES Cell line
Background strainFVB/NJcl
Appearance
Strain developmentW4 mESC were transformed with linearized pROSA-Pik3ca-H1047R or pROSA-Pik3ca-E545K plasmid; this plasmid was built from pBIGT and pROSA gene targeting vectors and contained the mouse Pik3ca cDNA with H1047R or E545K point mutation.
Strain descriptionSome mice develop blood vessel lesions.
Colony maintenance
ReferencesCancer Res., 1, 2706-2717 (2011). 21324922

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Pik3ca*E545K)Egan>targeted mutation 1, Sean E Egan

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pik3caphosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha6Pik3ca

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tpAtrimer of the SV40 polyadenylation sequence (tpA)6tpA

Ordering Information

Donor DNAadenovirus splice acceptor (SA), Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, trimer of the SV40 polyadenylation sequence (tpA), mouse Pik3ca E545K cDNA, bovine growth hormone polyadenylation sequence (bpA), mouse Gt(ROSA)26Sor genomic DNA
Research applicationCancer Research
Cre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must prior contact with the developer scientist(Dr. Sean E. Egan, segan@sickkids.ca, Hospital for Sick Children) and must obtain a use license.
DepositorYasushi Okazaki (Saitama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data