RBRC09450, B6-Gt(Rosa)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi>

RBRC No. RBRC09450
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6-Gt(Rosa)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi>
Former Common name RCL-G6-2A-mCh
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol F2A
Symbol name Foot-and-Mouth Disease Virus (FMDV) 2A peptide
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol mCherry
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol poly A
Symbol name polyadenylic acid additional signal
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
8 Symbol GCaMP6
Symbol name RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
3) The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR's permission.
4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2012 Dec 11; 7(12), e51286, 2012.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Shionogi & Co., Ltd.
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :