RBRC09486, B6.Cg-Xcr1<tm1Ksho>

RBRC No. RBRC09486
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Xcr1<tm1Ksho>
Former Common name XCR1-venus, XCR1-venus B6
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.
Strain description A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-venus mice were generated by knocking the gene encoding a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-venus mice, XCR1-expressing cells can be detected by venus expression.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol Xcr1
Symbol name chemokine (C motif) receptor 1
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form (form D).
2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.J. Immunol., 190, 6071-6082 (2013).
3. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
4. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp).
Additional information
1 Approval form Form V (English)
2 Mouse of the Month Jan 2016
3 Genotyping protocol <PCR>
Depositor Kaisho, Tsuneyasu (RIKEN Center for Integrative Medical Sciences (IMS)) Kaisho, Tsuneyasu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months