RBRC09657, B6.Cg-Clec4a4<tm1.1Ksat>

RBRC No. RBRC09657
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Clec4a4<tm1.1Ksat>
Former Common name Clec4a4-/- mice
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to C57BL/6J for 9 generations.
Strain description Clec4A4 (DCIR2) knockout mice. IRES-EGFP cassette was replaced with exon 1 and exon 2 of the Clec4A4 gene. No GFP expression was detected in the mice.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Clec4a4
Symbol name C-type lectin domain family 4, member a4
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Ires2
Symbol name internal ribosomal entry site (EMCV)
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Commun., 7, 11273 (2016).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a "MTA for Clec4a4-IRES2EGFP KI transgenic mice for Academic Research Purposes" specified by the DEPOSITOR and Inserm.
Additional information
1 MTA for Clec4a4-IRES2EGFP Knock-In Mice for Academic Research Purposes (English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor’s present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
2 Genotyping protocol <PCR>
Depositor Sato, Katsuaki (RIKEN Research Center For Allergy and Immunology) Sato, Katsuaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months