Strain Information | |
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Image | |
BRC No. | RBRC09665 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6(CB)-Gt(ROSA)26Sor<tm1.1(CAG-AKR1C3)Watk> |
Former Common name | hPGFS KI |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Kikuko Watanabe, Koshien University in 2015. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 and CBA mixed background. |
Strain description | Knock-in mice for overexpression of human AKR1C3. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-FAM213B-pA cassette into the mouse Rosa26 locus. A DNA fragment of loxP-CAT-FRT-PGK-neo-FRT-loxP was removed by crossing to B6;CBA-Tg(CAG-Cre)47Imeg. |
Colony maintenance | |
References | Prostaglandins Other Lipid Mediat., 68-69:401-407 (2002). 12432932 Genes Dev., 5, 1513-1523 (1991). 1653172 FEBS Lett., 462, 335-340 (1999). 10622721 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-AKR1C3)Watk>targeted mutation 1.1, Kikuko Watanabe Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter AKR1C3human ortholog mouse Akr1c18 (MGI: 2145420), aldo-keto reductase family 1, member C186AKR1C3CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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Donor DNA | マウス(Mus musculus) ROSA26, ヒト(Homo Sapiens) hPGFS,ウシ(Bos taurus) bovine growth hormone polyA signal, サイトメガロウィルス(cytomegalovirus) cytomegalovirus enhancer, ニワトリ(Gallus gallus) β-actin promoter, ウサギ(Oryctolagus cuniculus)β-globin exon, intron, ファージ P1 (Enterobacteria phage P1) Lox-P |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS lett., 462, 335-340 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested. |
Depositor | Kikuko Watanabe (Kansai Medical University) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |