Strain Data Sheet

RBRC09691

Strain Information

Image
BRC No.RBRC09691
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.C3-Tg(CAG-Il1rl1,CAG-GFP)23Tomi
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shin-ichi Tominaga and Kenji Kuroiwa, Jichi Medical University. DNA constructs (CAG-Il1rl1 and CAG-GFP) were injected to fertilized eggs derived from C3H/HeJ. Mice were backcrossed to BALB/c for 7 generations.
Strain descriptionTransgenic mice expressing GFP and a secreted form of Il1rl1 (interleukin 1 receptor-like 1; alternative name, ST2) under the control of CAG promoter. Hundreds ng/ml of ST2 can be detected in serum of transgenic mice. Related transgenic mouse lines are available as RBRC09692 and RBRC09693.
Colony maintenance
ReferencesJ. Biol .Chem., 275(21):15992-6001 (2000). 10821853
Eur. J. Immunol., 40, 2632-2642 (2010). 20662097

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFP(S65T/S147P)Green Fluorescent Protein (Aequorea victoria)UNGFP(S65T/S147P)CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il1rl1interleukin 1 receptor-like 1UNIl1rl1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mouse Il1rl1 cDNA, jellyfish GFP(S65T/S147P) cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the Dr. Tsukasa Ohmori, Jichi Medical University using the Approval Form.　The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorShin-ichi Tominaga (Jichi Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data