RBRC09714, B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>

RBRC No. RBRC09714
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>
Former Common name Chx10-IRES-CreERT2 KI
H-2 Haplotype No Data
ES cell line JM8A3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background.
Strain description IRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase.
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol Vsx2
Symbol name visual system homeobox 2
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 12
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months