RBRC09728, B6.Cg(C)-Hist1h2aa/Hist1h2ba<tm1Sis>

RBRC No. RBRC09728
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg(C)-Hist1h2aa/Hist1h2ba<tm1Sis>
Former Common name Th2a- Th2b-
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shunsuke Fujii, RIKEN in 2014. R1 ES cell line [(129X1/SvJ x 129S1)F1] was used. After BALB/cA congenic line had been established, this line was backcrossed to C57BL/6J for 5 generations. BALB/cA congenic line is available at RBRC09729.
Strain description A 3.6 kb region, which covers both Hist1h2aa and Hist1h2ba, was replaced with a neo cassette via homologous recombination. It is expected that male homozygotes are sterile and female homozygotes have low fertility.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Hist1
Symbol name histone cluster 1
Chromosome 13
Common name Th2a- Th2b-
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name No Data
Symbol description No Data
References No Data

Research applications Cell Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Ishii, Shunsuke (RIKEN)
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months