RBRC09730, B6.Cg-Braf<tm1Tumg>

RBRC No. RBRC09730
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Braf<tm1Tumg>
Former Common name Braf Q241R Neo/+ (C57BL/6 background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. Mice were maintained by sister-brother mating.
Strain description Knock-in mutants in which Braf-mutated protein (BrafQ241R) is expressed in a Cre-dependent manner. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. BrafQ241R heterozygous mice in the ICR genetic background are available as RBRC09731.
Health Report
Gene Details
Promoter No Data
1 Symbol Braf
Symbol name Braf transforming gene
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Human Molecular Genetics, 23, 6553-6566 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months