Strain Data Sheet

RBRC09746

Strain Information

Image
BRC No.RBRC09746
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Has1<tm1Nita>
Former Common nameHAS1 knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Naoki Itano and Koji Kimata, Aichi Medical University in 2000. E14 ES cell was used. C57BL/6N congenic line.
Strain descriptionHas1 (hyaluronan synthase1) knockout mice.
Colony maintenance
ReferencesCancer Res. 2010 Sep 15;70(18):7073-83. doi: 10.1158/0008-5472.CAN-09-4687. 20823158
J. Invest. Dermatol., 132, 198-207 (2012). 21850020

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Has1hyaluronan synthase 117Has1<tm1Nita>targeted mutation 1, Naoki Itano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal17

Ordering Information

Donor DNAMouse Phosphoglycerate kinase (PGK) promoter, E coli Neomycin resistance gene, SV40 PolyA signal, Mouse Has1 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorKoji Kimata (Aichi Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data