RBRC09758, B6;129P2-Chd8<tm1Kei>

RBRC No. RBRC09758
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Chd8<tm1Kei>
Former Common name Chd8/Duplin KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Deposited by Keiichi Nakayama, Medical Institute of Bioregulation, Kyusyu University in 2016. E14(129P2/OlaHsd) ES cell line was used. Mixed genetic background in C57BL/6 and 129P2.
Strain description Chd8 (chromodomain helicase DNA binding protein 8) knockout mice. Homozygous mutants are lethal. Heterozygous mutant displays autistic like behavior. All exons were replaced by loxP-neo cassette. Mouse strain carrying deletion of only long isoform of Chd8 (Chd8l) is available as RBRC09759.
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol Chd8
Symbol name chromodomain helicase DNA binding protein 8
Chromosome 14
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Mouse of the Month Nov 2018
2 Genotyping protocol <PCR>
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months