Strain Information | |
---|---|
Image | |
BRC No. | RBRC09904 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Has1<tm1Nita>Has2<tm1.1Nita> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Naoki Itano, Kyoto Sangyo Universit. RBRC09746 and RBRC09894 are crossed. |
Strain description | Double mutant of Has1 KO and floxed Has2. Single mutant of Has1 KO or floxed Has2 is available as RBRC09746 or RBRC09894, respectively. |
Colony maintenance | |
References | Cancer Res., 70(18), 7073-7083(2010). 20823158 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal17 |
Ordering Information | |
---|---|
Donor DNA | Mouse Phosphoglycerate kinase (PGK) promoter, E col. Neomycin resistance gene, SV40 PolyA signal, mouse Has1 genomic DNA, Phage P1 loxP sites, yeast FRT recombinase target site, mouse Has2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Naoki Itano (Kyoto Sangyo University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |