RBRC09909, C57BL/6N-Nalcn<em1Iiis>

RBRC No. RBRC09909
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6N-Nalcn<em1Iiis>
Former Common name Nalcn
H-2 Haplotype No Data
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development The EEG/EMG-based dominant screening of ENU-mutagenized mice yielded a mutant pedigree with a REMS abnormality, which we termed Dreamless (Drl). The founder of the pedigree showed a short REMS episode duration. Whole-exome sequencing combined with direct sequencing of candidate genes identified a heterozygous missense mutation in the Nalcn gene (chr14: 123515403). We then confirmed the causal relationship of the Nalcn gene mutation to the REMS phenotype by introducing the same nucleotide substitution in wild-type mice using the CRISPR/Cas9 system. CRISPR NalcnDrl/+ mice showed a short REMS episode duration, similar to the original Dreamless pedigree.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Nalcn
Symbol name sodium leak channel, non-selective
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 539, 378-383 (2016).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
Depositor Yanagisawa, Masashi (University of Tsukuba) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months