RBRC09930, C57BL/6J-Tg(CAG-GFP/LC3/RFP/LC3<*>deltaG)2Nmz

RBRC No. RBRC09930
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(CAG-GFP/LC3/RFP/LC3<*>deltaG)2Nmz
Former Common name GFP-LC3-RFP-LC3 delta G#2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background.
Strain description Transgenic mice expressing GFP-LC3 and RFP-LC3ΔG under the control of the CAG promoter. High expression was observed only in the skeletal muscle.
Health Report
Gene Details
Promoter No Data
1 Symbol Map1lc3b
Symbol name microtubule-associated protein 1 light chain 3 beta
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol RFP
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. 2. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Mol. Cell 64:835-849 (2016). 5. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Mizushima, Noboru (The University of Tokyo, Graduate School of Medicine) Mizushima, Noboru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months