Strain Information | |
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Image | |
BRC No. | RBRC09932 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | C57BL/6-Prm2<em2(S56D)Kyot> |
Former Common name | Prm2 S56D |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Katsuhiko Itoh, Graduate School of Medicine and Faculty of Medicine, Kyoto University in 2015. C57BL/6 genetic background. |
Strain description | A point mutation S56D was intrduced into the Prm2 (protamine 2) gene by the CRISPR/Cas9 technique. Homozygous mice are fertile. |
Colony maintenance | |
References | Sci. Signal., 12(574) (2019). 30914484 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin] |
Research application | |
Specific Term and Conditions | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Katsuhiko Itoh (Kyoto University) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |