Strain Information | |
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Image | |
BRC No. | RBRC10093 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Plcz1<em3(D210R)Osb> |
Former Common name | Plcz1<D210R/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kaori Nozawa and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Mutant harboring a point mutation at the Plcz1 gene (D210R) generated by the genome editing technique. Plcz1<em3(D210R)Osb>; D210R at Exon 6.atgactagggaggagcagagaccatttgtaggacagaggcaaagagagtgtttctatttcataaggctgtaagttcctaggatccaaaacttctctctatacagactggctcatccatttattttaaaagtctgaacattttgatcttattctacccatggttcatggtctagatatgacactattttgcctcccttttgctagTGCTCTTGTGAAAGGCTGCCGCTGTCTGGAAATTGAC(CGT)TGCTGGGATGGATCCCAAAATGAACCCATTGTGTACCATGGTTACACATTCACCAGCAAGCTTCTCTTCAAAACTGTGGTCCAAGCAATAAACAAGTATGCCTTTGTGgtatgtgtgcgtctccgatgcaggcatttaaagaatttactgctcccaaatgctatacagcaaggccttactttaaatctgtgagattttaacttttaagctatgattggcatcagaatccatgtaatatttagtagctaaaggtaggtttatcaaagtgaaaggtagtgagtggtcatgggaat |
Colony maintenance | |
References | Sci. Rep., 8(1):1315 (2018). doi: 10.1038/s41598-018-19497-6. 29358633 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Plcz1phospholipase C, zeta 16Plcz1<em3(D210R)Osb>endonuclease-mediated mutation 3, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Plcz1gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 2018 Jan 22;8(1):1315. doi: 10.1038/s41598-018-19497-6.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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