RBRC10246, B6.129P2-Avil<tm2(cre)Faw>

RBRC No. RBRC10246
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Avil<tm2(cre)Faw>
Former Common name Advillin-Cre mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development The line SHOULD be maintained as male heterozygous, homozygous or female advillin-Cre mice have leaky Cre expression.
E14 ES cells were used to generate this strain, and then mice were crossed to C57BL/6J.
Number of backcrossing to C57BL/6J:unknown.
Strain description Cre was knocked into exon 2 of the Avil locus.
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Sep 9, 2019 2-検 C
Jul 8, 2019 2-検 C
May 7, 2019 2-検 C
Mar 11, 2019 2-検 C
Jan 15, 2019 2-検 C
Nov 12, 2018 2-検 C

Gene Details
Promoter No Data
1 Symbol No Data
Symbol name HSV thymidine kinase polyA signal
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol Avil
Symbol name advillin
Chromosome 10
Common name No Data
Symbol description No Data
3 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 10
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name Bovine Growth Hormone polyA
Chromosome 10
Common name No Data
Symbol description No Data
5 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 10
Common name No Data
Symbol description No Data
6 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 10
Common name No Data
Symbol description No Data
Promoter mouse Ace promoter
7 Symbol Flp
Symbol name No Data
Chromosome 10
Common name No Data
Symbol description No Data
Promoter mouse Polr2a promoter
8 Symbol neo
Symbol name neomycin resistance gene
Chromosome 10
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci., U S A, 107(20), 9424-9429(2010).
The BIOLOGICAL RESOURCE is solely for research use by nonprofit institutes only. The RECIPIENT of a for-profit organization must first obtain a use license from the DEPOSITOR. Depositor shall notify RIKEN BRC of its written consent within (10) days of execution of any use license for the BIOLOGICAL RESOURCE. It shall be the sole responsibility of Depositor to obtain and enforce any use licenses.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Wang, Fan (Duke University) Wang, Fan
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months