Strain Data Sheet

RBRC10285

Strain Information

Image
BRC No.RBRC10285
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Syncrip<tm1Kmik>
Former Common nameSYNCRIP flox mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2009. TT2 ES cells derived from (C57BL/6 x CBA)F1 was used, and then the mice were backcrossed to C57BL/6 for 6 generations.
Strain descriptionSyncrip (synaptotagmin binding, cytoplasmic RNA interacting protein) floxed mice. Floxed Neo gene fragment was placed at intron 4 of the Syncrip gene and another loxP was placed at intron 2. TT2 ES cells derived from (C57BL/6 x CBA)F1 was used, and then the mice were backcrossed to C57BL/6 for 6 generations.
Colony maintenance
ReferencesJ. Biol. Chem. 275, 9823-9831 (2000). 10734137

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Syncripsynaptotagmin binding, cytoplasmic RNA interacting protein9Syncrip<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene9mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAP1 phageloxP sequence, Mouse PGK promoter, Escherichia coli neomycin-resistant gene (Pro/Neo/3'end), Mouse PGK pA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data