Strain Data Sheet

RBRC10289

Strain Information

Image
BRC No.RBRC10289
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Itpr2<tm1Kmik>
Former Common nameIP3R2 knockout mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. J1 ES cells derived from 129S4/SvJae were used. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations.
Strain descriptionItpr2 (inositol 1,4,5-trisphosphate receptor 2) KO mouse. A nls-LacZ gene fragment was inserted downstream of the translation initiation codon. Homozygous mutant exhibit decreased sweating and disturbed calcium signaling in sweat glands.
Colony maintenance
ReferencesScience, 309(5744) 2232-2234 (2005). 16195467
J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695
Sci. Rep., 11, 8150 (2021). 33854148

Health Report

Examination Date / Room / Rack2024/01/22Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Itpr2inositol 1,4,5-triphosphate receptor 26Itpr2<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Ordering Information

Donor DNAMouse PGK promoter, Escherichia coli neomycin-resistant gene, mouse PGK pA, SV40 large tumor nuclear location signal, Escherichia coli lacZ gene, mouse Itpr2 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Live miceLive mice
Strain AvailabilityLive mouse (more than 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data