Strain Information | |
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Image | |
BRC No. | RBRC10383 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Pip5k1a<tm1Tba> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Yasunori Kanaho and Takamitsu Unoki, University of Tsukuba in 2005. E14 ES cells derived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations. |
Strain description | )Pip5k1a (phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha) knockout mice. A floxed neo fragment replaced Exon 5 of the Pip5k1a gene. Homozygous males are infertile. |
Colony maintenance | |
References | Biol. Reprod., 86(5) 136, 1-12 (2012). 22321832 Sci. Rep., 7(1) 9438 (2017). 28842595 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pip5k1aphosphatidylinositol-4-phosphate 5-kinase, type 1 alpha3Pip5k1a<tm1Tba>targeted mutation 1, Tadashi Baba Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Pip5k1a genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 86(5) 136, 1-12 (2012). Sci. Rep., 7(1) 9438 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasunori Kanaho (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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No Data |