Strain Information | |
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Image | |
BRC No. | RBRC10449 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Hacd1<tm1Akih> |
Former Common name | Hacd1 KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Yusuke Ohno and Akio Kihara, Hokkaido University in 2010. ES cells (E14) derived from 129 were used. Mice were backcrossed to C57BL/6 for 16 generations. C57BL/6 congenic strain. |
Strain description | Hacd1 (3-hydroxyacyl-CoA dehydratase 1) knockout mice. Homozygous mutants exhibit reduced body and muscle weights. |
Colony maintenance | |
References | J. Biol. Chem., 292, 15538-15551 (2017). 28784662 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2E. coli EM7 promoter, Mouse Phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Bacteriophage P1 LoxP sites, Saccharomyces cerevisiae FRT sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Hacd1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Akio Kihara (Hokkaido University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |