Strain Data Sheet

RBRC10588

Strain Information

Image
BRC No.RBRC10588
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Pnldc1<em2Nkn>
Former Common namePnldcl_Exon 7-mut, Pnldc1-mut
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toru Nakano, Graduate School of Frontier Biosciences, Osaka University. C57BL/6 genetic background.
Strain descriptionPnldc1 (poly(A)-specific ribonuclease (PARN)-like domain containing 1) mutant mice. 836 bp insertion at Exon 7 generated with the Crispr/Cas9 technique and fertilized eggs derived from C57BL/6. Homozygous males are infertile, whereas homozygous females are fertile.
Colony maintenance
ReferencesEMBO Rep. 2018 Mar;19(3). pii: e44957. doi: 10.15252/embr.201744957. 29444933

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pnldc1poly(A)-specific ribonuclease (PARN)-like domain containing 117Pnldc1<em2Nkn>endonuclease-mediated mutation 2, Toru Nakano

Ordering Information

Donor DNAマウス Pnldc1
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T. PNLDCl, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 2018 Mar;l9(3). pii: e44957. doi: 10.15252/embr.201744957.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorToru Nakano (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data