RBRC10589, C57BL/6-Pnldc1<em3Nkn>

RBRC No. RBRC10589
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6-Pnldc1<em3Nkn>
Former Common name Pnldcl_del-14nt-Exon3, Pnldc1-KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Cas9 mRNA and each single-guide RNA (sgRNA) transcribed in vitro by the mMESSAGE mMACHINE T7 Ultra Kit (Ambion) were injected into C57BL6 zygotes. Two-cell-stage embryos were transplanted into pseudopregnant mice.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pnldc1
Symbol name poly(A)-specific ribonuclease (PARN)-like domain containing 1
Chromosome 17
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T. PNLDCl, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 2018 Mar;l9(3). pii: e44957. doi: 10.15252/embr.201744957.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months