Strain Data Sheet

RBRC10788

Strain Information

Image
BRC No.RBRC10788
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Eipr1<em1Card>
Former Common nameTssc1KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kimi Araki at Card, Kumamoto University in 2016. 6NK-7 ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N genetic background.
Strain descriptionEipr1 mutant mice with 13 bp deletion at Exon 1.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Eipr1EARP complex and GARP complex interacting protein 112Eipr1<em1Card>endonuclease-mediated mutation 1, Center for Animal Resources & Development

Ordering Information

Donor DNAAfter creating this strain using the CRISPR/Cas9 system, cas9 was not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 本系統を用いて公表された第一報の論文In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
DepositorKimi Araki (Kumamoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data