Strain Data Sheet

RBRC10935

Strain Information

Image
BRC No.RBRC10935
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6N.129-Brsk1<tm1Jrs>/Kcn
Former Common nameSAD-B KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeeposited by Keiko Nakanishi, Aichi Developmental Disability Center in 2019.
Strain descriptionBrsk1 knockout mice. Exon1 of the Brsk1 gene was replaced with a neo cassette. B6N congenic strain.
Colony maintenance
ReferencesScience. 2005 DOI:10.1126/science.1107403 15705853
Cereb Cortex. 2018 DOI:10.1093/cercor/bhy253 30307479

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Brsk1BR serine/threonine kinase 17Brsk1<tm1Jrs>targeted mutation 1, Joshua R Sanes

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 loxP sites, mouse PGK promoter, E. coli Neomycin resistant gene, mouse Brsk1 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science. 2005 DOI:10.1126/science.1107403, Cereb Cortex. 2018 DOI:10.1093/cercor/bhy253
DepositorKeiko Nakanishi (Aichi Developmental Disability Center Central Hospital)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data