RBRC00437, B6.129P2-Prnp<tm1Ito>/Rbrc

RBRC No. RBRC00437
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.129P2-Prnp<tm1Ito>/Rbrc
Former Common name Prnp-/- mouse, Prion protein (PrP) Knockout mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Takashi Yokoyama, National Institute of Animal Health and Shigeyoshi Itohara, RIKEN BSI in 2001.
C57BL/6J background.
Strain description PrP, the prion protein, plays a central role in the pathogenesis of transmissible neurodegenerative disorders such as scrapie or BSE. Homozygous mutant mice show the absence of cellular isoform of prion protein. Aged homozygous mice exhibit tremor and ataxia.
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Aged mice exhibit tremor and ataxia.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol PrnP
Symbol name prion protein
Chromosome 2
Common name PrP, PrPC, Sinc, +Prnp, CD230, PrPSc, Prn-i, Prn-p, PrP
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References J Biol Chem. 2001 Apr 6;276(14):11265-71. Epub 2001 Jan 10.
Nature. 1999 Jul 15;400(6741):225-6.

In vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies.
In vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies.
Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature. 1999 Jul 15;400(6741):225-6.
Additional information
1 Genetic Background
2 Genotyping protocol <PCR>
Depositor Onodera, Takashi (The University of Tokyo) Onodera, Takashi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
Cutting Edge: Brucella abortus Exploits a Cellular Prion Protein on Intestinal M Cells as an Invasive Receptor. J Immunol.189(4): 1540-4 (2012).(22772447)
Nakato G, Hase K, Suzuki M, Kimura M, Ato M, Hanazato M, Tobiume M, Horiuchi M, Atarashi R, Nishida N, Watarai M, Imaoka K, Ohno H.