RBRC01161, C57BL/6-Tg(CAG-EEA1/EGFP)1Tssk/TsskRbrc

RBRC No. RBRC01161
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(CAG-EEA1/EGFP)1Tssk/TsskRbrc
Former Common name EGFP-EEA1-1 transgenic mouse
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Sasaki (2002). The EGFP-EEA1 plasmid was injected into the pronucleous of C57BL/6JJcl fertilized eggs.
Strain description C57BL/6J-Tg(CAG-EEA1/EGFP)1Tssk. This transgenic mouse expresses the GFP and EEA1 fusion gene under the control of the CAG promoter.
Colony maintenance Sibling Mating
Health Report No Data
Gene Details
Promoter No Data
1 Symbol EEA1
Symbol name Early endosome antigen 1
Chromosome UN
Common name A430109M19Rik, B230358H09Rik, ZFYVE2
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cell Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, Dr. Sasaki T.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Sasaki, Takehiko (Akita University Faculty of Medicine) Sasaki, Takehiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months