Strain Data Sheet

RBRC01192

Strain Information

Image
BRC No.RBRC01192
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Meis3<tm1Ngc>/Rbrc
Former Common nameMeis3 Knockout mice
H-2 Haplotype
ES Cell lineJR2448 [129S3/SvImJ]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masami Fujita and Neal G. Copeland in National Cancer Institute in 1997. Exon 9 of Meis3 gene were replaced with lacZ, PGK-neo. C57BL/6 and 129 mixed backgrond.
Strain descriptionMesi3 knockout mice. Homozygous mutant mice are embryonic lethal, but are born alive only occasionally.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Meis3Meis homeobox 37Meis3<tm1Ngc>targeted mutation 1, Neal G CopelandMrg2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli lacZ, E. coli neo, mouse myeloid ecotropic viral integration site-related gene 2 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
DepositorTakuro Nakamura (Japanese Foundation for Cancer Research)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data