RBRC01254, B6;129S4-Cnot7<tm1Tno>/Rbrc

RBRC No. RBRC01254
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4-Cnot7<tm1Tno>/Rbrc
Former Common name Cnot7 Knockout mouse
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Donor strain 129S4/SvJae via J1 ES cell line
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Ryoji Yao, Tadashi Yamamoto, and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. An exon of Cnot7 subcloned the 18-kb genomic DNA fragment, was replaced by the insertion of a LacZ and a floxed neo cassette. The floxed neo cassette was excised in this strain. This strain originated on a B6;129 background.
Strain description B6;129S4-Cnot7<tm1Tno>. Cnot7 gene (CCR4-NOT transcription complex. subunit 7) is essential for spermatogenesis. Cnot7 deficient male mice are sterile owing to oligo-astheno-teratozoospermia, impaired sperm motility, unsynchronized spermatid maturation, and Sertoli cell abnormalities. This knockout mouse strain is a powerful tool for understanding the mechanisms underlying spermatogenesis.
Colony maintenance Heterozygote x Wild-type
Homozygous mutant males are sterile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cnot7
Symbol name CCR4-NOT transcription complex, subunit 7
Chromosome 8
Common name Caf1
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 8
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 8
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
References Nat Genet. 2004 May;36(5):528-33. Epub 2004 Apr 25.

Oligo-astheno-teratozoospermia in mice lacking Cnot7, a regulator of retinoid X receptor beta.
Research applications Cell Biology Research,
Genetics Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature Genetics, 36, 528-533 (2004).
It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications.
The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Noda, Tetsuo (The CANCER INSTITUTE of the Japanese Foundation for Cancer Research) Noda, Tetsuo
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months