Strain Data Sheet

RBRC01300

Strain Information

Image
BRC No.RBRC01300
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameD2.Cg-Prkdc<scid>/Rbrc
Former Common nameDBA/2-scid/+, DBA/2-scid mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Mie University Graduate School of Medicine. The original scid mice were provided by Ohtura Niwa, Radiation Biology Center, Kyoto University (C57BL/6-scid p<un>). The mutant mice were backcrossed to DBA/2Cr and C57BL/6NCr (RBRC01300 and RBRC01346, respectively).
Strain descriptionscid, severe combined immune deficiency mice. Homozygous mutant mice lack both functional T and B cells, resulting in severe combined immunodeficiency. DBA/2 background (RBRC01300), C57BL/6 background (RBRC01346). We do not carry out a detection of leaky mice by measuring serum Ig level (PCR genotying of scid mutation only).
Colony maintenanceHomozygote x Homozygote [or Crossing to DBA/2CrSlc]
ReferencesProc. Natl. Acad. Sci. USA, 94, 2438-2443 (1997). 9122213
Radiat. Res., 157, 661-667(2002). 12005545

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<scid>severe combined immune deficiency

Ordering Information

Donor DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for basic research, shall not use for commercial use.
DepositorKagemasa Kuribayashi (Mie University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data