Strain Information | |
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Image | |
BRC No. | RBRC01886 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | B6.Cg-Aff3<Tn(sb-lacZ,GFP)PV516Jtak>/JtakRbrc |
Former Common name | Gene trap of Laf4 (lymphoid nuclear protein related to AF4), PV516 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Junji Takeda at Osaka University in 2005. |
Strain description | This strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
References | Nat. Methods, 2, 763-769 (2005). 16179923 Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Aff3AF4/FMR2 family, member 31Aff3<Tn(sb-lacZ,GFP)PV384Jtak>gene trap trap lacZ/GFP PV384, Junji Takeda Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hprtmouse Hprt splice donor (SD)1Hprt |
Ordering Information | |
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Donor DNA | salmonid Sleeping Beauty transposon, mouse Hprt splice donor, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP cDNA, polyA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Junji Takeda (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |