Strain Data Sheet

RBRC01891

Strain Information

Image
BRC No.RBRC01891
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Clec10a<tm1Hed>/TatsRbrc
Former Common namemMgl1 knockout mice (C57BL/6J)
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6J
Appearance
Strain developmentDeveloped by Stephen M Hedrick, University of California. R1 ES cells were used to generate the knockout mice. R RBRC01891: backcrossed to C57BL/6J over 8 times. RBRC01892: backcrossed to C57BL/6J for 8 times, and then backcrossed to BALB/c over 6 times.
Strain descriptionMgl1 gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01891) and BALB/c mixed background (RBRC01892). Homozygous mutant mice were viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesMol. Cell. Biol., 22, 5173-5181 (2002). 12077344

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Clec10amacrophage galactose N-acetyl-galactosamine specific lectin 111Clec10a<tm1Hed>targeted mutation 1, Stephen M HedrickMgl1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Clec10a genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTatsuro Irimura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data