RBRC01922, B6;129-Zeb1<tm1Yhi>

RBRC No. RBRC01922
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Zeb1<tm1Yhi>
Former Common name δ EF1 [Delta]C-fin, δ EF1 [Delta]C727
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 11 times.
Strain description Zeb1 gene knockout mice (deltaEF1 deltaC727). Exon 6 of the Zeb1 was replaced with a neo cassette. In the genetic background of inbred mouse strains, homozygous mutant mice die after birth. While about 20-30% of homozygous mice survive to adulthood in the mixed genetic background.
Health Report
Gene Details
Promoter No Data
1 Symbol Zeb1
Symbol name zinc finger E-box binding homeobox 1
Chromosome 18
Common name [delta]EF1, 3110032K11Rik, AREB6, MEB1, Nil2, Nil2, Tcf18, Tcf8, ZEB, Zfhep, Zfx1a
Symbol description No Data
Promoter SV40 early promoter and herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 18
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1997 Apr 21;185(8):1467-79.

Impairment of T cell development in deltaEF1 mutant mice.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 185, 1467-1480 (1997).
The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Higashi, Yujiro (Graduate School of Frontier Biosciences, Osaka University) Higashi, Yujiro
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months