Strain Data Sheet

RBRC02150

Strain Information

Image
BRC No.RBRC02150
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Phgdh<tm1Bsi>
Former Common namePhgdh KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Yoshida, Furuya and Hirabayashi at RIKEN BSI in 2004.
Strain descriptionPhgdh, 3-phosphoglycerate dehydrogenase is a necessary enzyme for de novo L-serine biosynthesis via the phosphorylated pathway. Homozygous null mutant mice died after d.p.c 13.5. The Phgdh null embryos exhibited small bodies with apparent brain and limb bud abnormalities.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesJ. Biol. Chem., 279, 3573-3577 (2004). 14645240

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Phgdh3-phosphoglycerate dehydrogenase3Phgdh<tm1.1Shfu>targeted mutation 1.1, Shigeki Furuya3-PGDH, 3PGDH, A10, PGAD, PGD, PGDH, SERA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse 3-Phosphoglycerate dehydrogenase (Phgdh) genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 279, 3573-3577 (2004).1.The RECIPIENT agrees to use BIOLOGICAL RESOURCE only for academic research which is expected to be publicized in scientific papers.
2.The RECIPIENT will obtain a prior written consent on the use of BIOLOGICAL RESOURCE from the DEPOSITOR/DEVELOPER. At the same time, the RECIPIENT will give the DEPOSITOR/DEVELOPER the details of the research project using BIOLOGICAL RESOURCE for the DEPOSITOR/DEVELOPER to determine whether research collaboration is appropriate.
3. If the DEPOSITOR/DEVELOPER determines that the informed research project requires collaboration, a separate agreement will be formulated by both parties to establish rules such as handling of the research results.
4.If the DEPOSITOR/DEVELOPER determines that the informed research project does not require collaboration, the RECIPIECT agrees to cite the literature(s) designated by the DEPOSITOR/DEVELOPER when publicizing the research results obtained from the use of BIOLOGICAL RESOURCE, and to discuss with the DEPOSITOR/DEVELOPER when application for any patents is considered.
5.If the RECIPIENT wants to crossbreed BIOLOGICAL RESOURCE with other genetically engineered mice, the RECIPIENT will obtain a prior written consent from the DEPOSITOR/DEVELOPER.
6.The RECIPIENT will not distribute the BIOLOGICAL RESOURCE to any other individual or entity.
DepositorYoshio Hirabayashi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data