RBRC02655, B6;129-Xist<tm5Sado>

RBRC No. RBRC02655
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6;129-Xist<tm5Sado>
Former Common name Xist; B6.Cg-Xist
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takashi Sado, National Institute of Genetics in 2007. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).
Strain description Xist<delta> mice. The portion of exon 1 encoding the A repeat was deleted. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenance Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Xist
Symbol name inactive X specific transcripts
Chromosome X
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome X
Common name No Data
Symbol description No Data
References Development. 2009 Jan;136(1):139-46. Epub 2008 Nov 26.

A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Feb 2009
3 Genotyping protocol <PCR>
Depositor Sado, Takashi (Medical institute of bioregulation, Kyushu University)
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice. PLoS Genet.12(10): e1006375. (2016).(27788132)
Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H.