RBRC02759, B6.Cg-Atg7<tm1Tchi>

RBRC No. RBRC02759
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Atg7<tm1Tchi>
Former Common name C57BL/6-Atg7 flox/flox
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masaaki Komatsu and Tomoyuki Chiba, Tokyo Metropolitan Institute of Medical Science. The targeting construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mice were backcrossed to C57BL/6 over 5 times.
Strain description Atg7, autophagy-related 7 gene floxed mice. A targeting vector was constructed by insertion of a loxP site within introns 13 and 14. Exon 14 was fused to a cDNA fragment encoded by exons 15, 16 and 17 and polyA after the stop codon. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Conditional Atg7 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Atg7
Symbol name autophagy-related 7 (yeast)
Chromosome 6
Common name 1810013K23Rik, Apg7l
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References J Cell Biol 2005 May 9;169(3):425-34. Epub 2005 May 2.

Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Cell Blol., 169, 425-434 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Jun 2012, Mouse of the Month Oct 2016
3 Genotyping protocol <PCR>
Depositor Komatsu, Masaaki (Tokyo Metropolitan Institute of Medical Science) Komatsu, Masaaki
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res. (2014).(24853953)
Wang H, Wan H, Li X, Liu W, Chen Q, Wang Y, Yang L, Tang H, Zhang X, Duan E, Zhao X, Gao F, Li W.
Hif-2 alpha promotes degradation of mammalian peroxisomes by selective autophagy. Cell Metab.20(5): 882-97 (2014).(25440060)
Walter KM, Sch nenberger MJ, Tr tzm ller M, Horn M, Els sser HP, Moser AB, Lucas MS, Schwarz T, Gerber PA, Faust PL, Moch H, K feler HC, Krek W, Kovacs WJ.
Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice. Cell Death Dis6: e1589 (2015).(25590799)
Song ZH, Yu HY, Wang P, Mao GK, Liu WX, Li MN, Wang HN, Shang YL, Liu C, Xu ZL, Sun QY, Li W.
Sexual dimorphism in activation of placental autophagy in obese women with evidence for fetal programming from a placenta-specific mouse model. Autophagy12(5): 752-69. (2016).(26986453)
Muralimanoharan S, Gao X, Weintraub S, Myatt L, Maloyan A.
Vitrification, in vitro fertilization, and development of Atg7 deficient mouse oocytes. Clin. Exp. Reprod. Med.43(1): 9-14 (2016).(27104152)
Bang S, Lee GK, Shin H, Suh CS, Lim HJ.
Cartilage-Specific Autophagy Deficiency Promotes ER Stress and Impairs Chondrogenesis in PERK-ATF4-CHOP-Dependent Manner. J. Bone Miner. Res. (2017).(28304100)
Kang X, Yang W, Feng D, Jin X, Ma Z, Qian Z, Xie T, Li H, Liu J, Wang R, Li F, Li D, Sun H, Wu S.
Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7. Mol. Vis.23: 228-241 (2017).(28465655)
Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY.