Strain Data Sheet

RBRC02857

Strain Information

Image
BRC No.RBRC02857
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Vps52<tm1Kab>/20Rbrc
Former Common nameVps52 #20(Acc.No.CDB0767K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).
Strain descriptionVps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesCell Rep., 2(5) 1363-74 (2012). 23142660

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Vps52VPS52 GARP complex subunit17Vps52<tm1Kab>targeted mutation 1, Kuniya Abe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin17HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence17lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNANeo:大腸菌由来、lox71,loxP:P1ファージ由来、frt:酵母菌由来、PGKプロモーター,PGK 3’end:マウス由来、rabbit beta-globin 3’end:ウサギ由来
Research application
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data