Strain Data Sheet

RBRC02868

Strain Information

Image
BRC No.RBRC02868
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAT-RhoK DN)4-11Koba
Former Common nameCAT-RhoA-K DN/4-11
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine at 1998. The transgene containing CMV promoter and bovine Rho-K DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionTransgenic mice conditionally expressed a dominant-negative form for RhoK by using the Cre-loxP system. The Rho GTPase is known to regulate diverse cellular processes, including cell morphology, cell motility, adhesion, and apoptosis. The activity of RhoK in these transgenic mice is suppressed in developing motor neurons by crossing with dopamine beta-hydroxylase promotor (DBH)-cre transgenic mice. This strain are useful for elucidation of the role of the Rho sigaling pathway in various tissues with tissue-specific cre Tg mice.
Colony maintenance
ReferencesJ. Neurosci., 24, 3480-3488 (2004). 15071095

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rhokras homolog gene family, member A (bovine)UNRhok

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNE. coliCMV promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
myc-tagmyc-epitope tagUNmyc-tag

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA signalUNpoly ASV40

Ordering Information

Donor DNACytomegalovirus CMV promoter, E. coli Chloramphenicol acetyl transferase, bovine Rho-kinase, SV40 polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 24, 3480-3488 (2004).
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data